Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material.
نویسندگان
چکیده
A differential agar medium for the identification of Ureaplasma urealyticum in primary cultures of clinical specimens is described. The differential medium (no. A7) is specific for the identification of U. urealyticum and other members of the genus Ureaplasma. Large-colony, classical Mycoplasma and Acholeplasma species and Proteus L colonies are unreactive on this differential medium. The medium incorporates the biochemical principle of the direct spot test for urease in colonies of Ureaplasma and contains added urea and a sensitive indicator of ammonia, manganous sulfate. Ureaplasma colonies on this medium are identified as dark golden-brown or rich deep-brown colonies, in sharp contrast to the light background of the medium, when viewed by direct transmitted illumination under the low power of the microscope.
منابع مشابه
Unusual colonies of Ureaplasma urealyticum (T mycoplasmas) in primary agar cultures of certain urine specimens.
The existence of unusual colonies of Ureaplasma urealyticum (T mycoplasmas) in primary agar cultures of certain urine specimens is reported and their morphology is illustrated.
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In this study, we evaluated the usefulness of a culture system used in our laboratory for the detection of Ureaplasma urealyticum in the endotracheal aspirates of neonates. Ureaplasmal broth was used to enhance the growth of U. urealyticum followed by observation of colonies on A7 agar. Of the 68 samples of endotracheal aspirates tested, 60 gave positive indication of urease activity by the bro...
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OBJECTIVE The purpose of this study was to examine the role of the genital mycoplasmas Mycoplasma hominis and Ureaplasma urealyticum as causes of bacteremia in a tertiary referral obstetrical, gynecological, and neonatal intensive care facility, over a period of 12 years from 1983 to 1994 inclusively. METHODS All clinically significant blood cultures were reviewed and the percentage of septic...
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ورودعنوان ژورنال:
- Journal of clinical microbiology
دوره 3 6 شماره
صفحات -
تاریخ انتشار 1976